Journal: Virology

Article Title: 17β-Estradiol inhibits HIV-1 by inducing a complex formation between β-catenin and estrogen receptor α on the HIV promoter to suppress HIV transcription.

doi: 10.1016/j.virol.2013.05.027

Figure Lengend Snippet: Fig. 7. Tethering of ERα, β-catenin, and TCF-4 at -143 site on the HIV LTR. Jurkat cells were transfected with wild-type (WT) LTR or LTR deleted in -143 (LTR Δ −143, deletion from −143 to −136 nt from +1 site) with or without ERα plasmid. ChIP was performed with either pull down with ERα antibody (a) or β-catenin or TCF-4 antibodies (b) and DNA amplified for HIV LTR. Data represent fold change in comparison to IgG of at least two independent experiments. Asterisks denote po0.05 in comparison to IgG determined by Student T-test.

Article Snippet: Jurkat cells were transfected with 0.5 μg ERα expression plasmid (pEGFP-C1ER alpha, Addgene, Cambridge, MA), 0.5 μg of either the WT-LTR or Δ-143-LTR previously described (Henderson et al., 2012) using Lipofectamine 2000 (Invitrogen).

Techniques: Transfection, Plasmid Preparation, Comparison

Journal: Planta medica

Article Title: Estrogenic Activity of Hyperforin in MCF-7 Human Breast Cancer Cells Transfected with Estrogen Receptor.

doi: 10.1055/s-0042-112594

Figure Lengend Snippet: Fig. 1 Effects of St Johnʼs wort (SJW) extract, hyperforin (HF), and 17β- estradiol (17β-E2) on ERE-luciferase activity in MCF-7 cells. The luciferase activities in MCF-7 cells transfected with ERα (A, C) or ERβ (B, D) and an ERE reporter plasmid were measured after treatment with 0.2 to 20 µg/mL SJW extract (A, B) or 10−5 to 10 µM HF (C, D). A positive control was prepared by treating the cells with 10−2 µM 17β-E2. Relative luciferase activities were calculated as percentages of the induced luminance relative to control after normalization to the Renilla luciferase activity. Bars represent the averages of triplicate determinations. Asterisks indicate significant differences from the control (Ctrl) determined using Dunnettʼs multiple comparison t-test (*p < 0.05 and *** p < 0.001).

Article Snippet: Briefly, Opti-MEM was used to dilute ERα (pEGFP‐C1-ERα, Addgene), ERβ (pcDNA Flag ERβ, Addgene), ERE (3× ERE TATA luc, Addgene), and Renilla luciferase (pRL-SV40, Promega) prior to the addition of the transfection reagent.

Techniques: Luciferase, Activity Assay, Transfection, Plasmid Preparation, Positive Control, Control, Comparison